Description: Rabbit polyclonal antibody to POLR3H Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human POLR3H. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: POLR3H Alternative Names: KIAA1665; RPC8; DNA-directed RNA polymerase III subunit RPC8; RNA polymerase III subunit C8; DNA-directed RNA polymerase III subunit H; RNA polymerase III subunit 22.9 kDa subunit; RPC22.9Entrez Gene (Human): 171568Entrez Gene (Mouse) : 78929SwissProt (Human): Q9Y535SwissProt (Mouse) : Q9D2C6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of POLR3H expression in mouse liver (A) whole cell lysates.
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Immunohistochemical analysis of POLR3H staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.