Description: Rabbit polyclonal antibody to ERK1/2 (Phospho-T202/Y204) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T202/Y204 of human ERK1/2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MAPK1; MAPK3 Alternative Names: MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p44-ERK1; MAPK1; ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2Entrez Gene (Human): 5595; 5594Entrez Gene (Mouse) : 26417; 26413Entrez Gene (Rat) : 50689; 116590SwissProt (Human): P27361; P28482SwissProt (Mouse) : Q63844; P63085SwissProt (Rat) : P21708; P63086Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ERK1/2 (Phospho-T202/Y204) expression in A549 (A), Panc1 (B), rat lung (C), AML12 (D) whole cell lysates.
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Immunohistochemical analysis of ERK1/2 (Phospho-T202/Y204) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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