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Anti-IL-6 Antibody CPA4914
  • Western blot analysis of IL-6 expression in Hela (A), U87MG (B), HuT78 (C), A549 (D), MCF7 (E) whole cell lysates.
  • Immunohistochemical analysis of IL-6 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blot analysis of IL-6 expression in U87MG (A), mouse brain (B), mouse kidney (C), rat kidney (D) whole cell lysates.
Product NameAnti-IL-6 Antibody
Cat No: CPA4914
Source: Rabbit
Reactivity: H, M, R, Mk
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to IL-6
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IL-6. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200)
Gene Symbol: IL6
Alternative Names: IFNB2; Interleukin-6; IL-6; B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2
Entrez Gene (Human): 3569
Entrez Gene (Mouse) : 16193
Entrez Gene (Rat) : 24498
SwissProt (Human): P05231
SwissProt (Mouse) : P08505
SwissProt (Rat) : P20607
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of IL-6 expression in Hela (A), U87MG (B), HuT78 (C), A549 (D), MCF7 (E) whole cell lysates.
  • Immunohistochemical analysis of IL-6 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blot analysis of IL-6 expression in U87MG (A), mouse brain (B), mouse kidney (C), rat kidney (D) whole cell lysates.
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