Description: Rabbit polyclonal antibody to FADD (Phospho-S194) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S194 of human FADD protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: FADD Alternative Names: MORT1; FAS-associated death domain protein; FAS-associating death domain-containing protein; Growth-inhibiting gene 3 protein; Mediator of receptor induced toxicity; Protein FADDEntrez Gene (Human): 8772Entrez Gene (Mouse) : 14082SwissProt (Human): Q13158SwissProt (Mouse) : Q61160Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of FADD (Phospho-S194) expression in mouse lung (A), rat lung (B) whole cell lysates.
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  Immunohistochemical analysis of FADD (Phospho-S194) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.