Description: Rabbit polyclonal antibody to LKB1 (Phospho-T189) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T189 of human LKB1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: STK11 Alternative Names: LKB1; PJS; Serine/threonine-protein kinase STK11; Liver kinase B1; LKB1; hLKB1; Renal carcinoma antigen NY-REN-19Entrez Gene (Human): 6794Entrez Gene (Mouse) : 20869SwissProt (Human): Q15831SwissProt (Mouse) : Q9WTK7SwissProt (Rat) : D4AE59Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of LKB1 (Phospho-T189) expression in AML12 (A), PC12 (B), A375 (C), Panc1 (D), SHSY5Y (E) whole cell lysates.
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Immunohistochemical analysis of LKB1 (Phospho-T189) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.