Description: Rabbit polyclonal antibody to SREBP1 (Phospho-S439) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S439 of human SREBP1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SREBF1 Alternative Names: BHLHD1; SREBP1; Sterol regulatory element-binding protein 1; SREBP-1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1Entrez Gene (Human): 6720Entrez Gene (Mouse) : 20787Entrez Gene (Rat) : 78968SwissProt (Human): P36956SwissProt (Mouse) : Q9WTN3SwissProt (Rat) : P56720Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SREBP1 (Phospho-S439) expression in H460 (A) whole cell lysates.
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Immunohistochemical analysis of SREBP1 (Phospho-S439) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.