Description: Rabbit polyclonal antibody to SKIL Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human SKIL. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SKIL Alternative Names: SNO; Ski-like protein; Ski-related oncogene; Ski-related proteinEntrez Gene (Human): 6498Entrez Gene (Mouse) : 20482SwissProt (Human): P12757SwissProt (Mouse) : Q60665Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SKIL expression in HEK293T (A), H446 (B), rat liver (C) whole cell lysates.
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Immunohistochemical analysis of SKIL staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.