Description: Rabbit polyclonal antibody to SAP49 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SAP49. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SF3B4 Alternative Names: SAP49; Splicing factor 3B subunit 4; Pre-mRNA-splicing factor SF3b 49 kDa subunit; SF3b50; Spliceosome-associated protein 49; SAP 49Entrez Gene (Human): 10262Entrez Gene (Mouse) : 107701Entrez Gene (Rat) : 295270SwissProt (Human): Q15427SwissProt (Mouse) : Q8QZY9SwissProt (Rat) : Q6AYL5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SAP49 expression in LO2 (A), A549 (B), HEK293T (C), CT26 (D), PC12 (E) whole cell lysates.
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  Immunohistochemical analysis of SAP49 staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.