Description: Rabbit polyclonal antibody to S6K1 (Phospho-T229) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T229 of human S6K1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: RPS6KB1 Alternative Names: STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KAEntrez Gene (Human): 6198Entrez Gene (Mouse) : 72508Entrez Gene (Rat) : 83840SwissProt (Human): P23443SwissProt (Mouse) : Q8BSK8SwissProt (Rat) : P67999Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of S6K1 (Phospho-T229) expression in HT1080 (A), LOVO (B), NIH3T3 (C) whole cell lysates.
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  Immunohistochemical analysis of S6K1 (Phospho-T229) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of S6K1 (Phospho-T229) staining in HT1080 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).