Description: Rabbit polyclonal antibody to c-Raf/B-RAF (Phospho-T491/599) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T491/599 of human c-Raf/B-RAF protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: RAF1; BRAF Alternative Names: RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1Entrez Gene (Human): 5894; 673Entrez Gene (Mouse) : 110157; 109880Entrez Gene (Rat) : 24703SwissProt (Human): P04049; P15056SwissProt (Mouse) : Q99N57; P28028SwissProt (Rat) : P11345Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of c-Raf/B-RAF (Phospho-T491/599) expression in HEK293T (A), HeLa (B) whole cell lysates.
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  Immunohistochemical analysis of c-Raf/B-RAF (Phospho-T491/599) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.