Description: Rabbit polyclonal antibody to RAD51A (Phospho-Y315) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y315 of human RAD51A protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: RAD51 Alternative Names: RAD51A; RECA; DNA repair protein RAD51 homolog 1; HsRAD51; hRAD51; RAD51 homolog AEntrez Gene (Human): 5888Entrez Gene (Mouse) : 19361SwissProt (Human): Q06609SwissProt (Mouse) : Q08297Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RAD51A (Phospho-Y315) expression in HeLa UV-treated (A), HEK293T UV-treated (B) whole cell lysates.
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  Immunohistochemical analysis of RAD51A (Phospho-Y315) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.