Description: Rabbit polyclonal antibody to PKC beta (Phospho-S661) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S661 of human PKC beta protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PRKCB Alternative Names: PKCB; PRKCB1; Protein kinase C beta type; PKC-B; PKC-betaEntrez Gene (Human): 5579Entrez Gene (Mouse) : 18751Entrez Gene (Rat) : 25023SwissProt (Human): P05771SwissProt (Mouse) : P68404SwissProt (Rat) : P68403Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PKC beta (Phospho-S661) expression in mouse brain (A) whole cell lysates.
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  Immunohistochemical analysis of PKC beta (Phospho-S661) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.