Description: Rabbit polyclonal antibody to MYPT1 (Phospho-T853) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T853 of human MYPT1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PPP1R12A Alternative Names: MBS; MYPT1; Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunitEntrez Gene (Human): 4659Entrez Gene (Mouse) : 17931Entrez Gene (Rat) : 116670SwissProt (Human): O14974SwissProt (Mouse) : Q9DBR7SwissProt (Rat) : Q10728Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MYPT1 (Phospho-T853) expression in C6 (A), MCF7 (B) whole cell lysates.
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  Immunohistochemical analysis of MYPT1 (Phospho-T853) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.