Description: Rabbit polyclonal antibody to PDPK1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PDPK1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PDPK1 Alternative Names: PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1Entrez Gene (Human): 5170Entrez Gene (Mouse) : 18607Entrez Gene (Rat) : 81745SwissProt (Human): O15530SwissProt (Mouse) : Q9Z2A0SwissProt (Rat) : O55173Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PDPK1 expression in Hela (A), PC12 (B) whole cell lysates.
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Immunohistochemical analysis of PDPK1 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.