Description: Rabbit polyclonal antibody to MDM2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MDM2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: MDM2 Alternative Names: E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2Entrez Gene (Human): 4193Entrez Gene (Mouse) : 17246SwissProt (Human): Q00987SwissProt (Mouse) : P23804Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MDM2 expression in mouse spleen (A), rat lung (B), rat spleen (C) whole cell lysates.
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  Immunohistochemical analysis of MDM2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.