Description: Rabbit polyclonal antibody to ERK7 (Phospho-T175/Y177) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T175/Y177 of human ERK7 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: MAPK15 Alternative Names: ERK7; ERK8; Mitogen-activated protein kinase 15; MAP kinase 15; MAPK 15; Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8Entrez Gene (Human): 225689SwissProt (Human): Q8TD08Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ERK7 (Phospho-T175/Y177) expression in Jurkat (A) whole cell lysates.
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  Immunohistochemical analysis of ERK7 (Phospho-T175/Y177) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.