Description: Rabbit polyclonal antibody to MKK7 (Phospho-S271) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S271 of human MKK7 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: MAP2K7 Alternative Names: JNKK2; MEK7; MKK7; PRKMK7; SKK4; Dual specificity mitogen-activated protein kinase kinase 7; MAP kinase kinase 7; MAPKK 7; JNK-activating kinase 2; MAPK/ERK kinase 7; MEK 7; Stress-activated protein kinase kinase 4; SAPK kinase 4; SAPKK-4; SAPKK4; c-Jun N-terminal kinase kinase 2; JNK kinase 2; JNKK 2Entrez Gene (Human): 5609Entrez Gene (Mouse) : 26400Entrez Gene (Rat) : 363855SwissProt (Human): O14733SwissProt (Mouse) : Q8CE90SwissProt (Rat) : Q4KSH7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MKK7 (Phospho-S271) expression in LOVO EGF-treated (A), NIH3T3 EGF-treated (B) whole cell lysates.
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  Immunohistochemical analysis of MKK7 (Phospho-S271) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.