Description: Rabbit polyclonal antibody to LCK (Phospho-Y505) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y505 of human LCK protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: LCK Alternative Names: Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCKEntrez Gene (Human): 3932Entrez Gene (Mouse) : 16818Entrez Gene (Rat) : 313050SwissProt (Human): P06239SwissProt (Mouse) : P06240SwissProt (Rat) : Q01621Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of LCK (Phospho-Y505) expression in Jurkat (A), HeLa UV-treated (B) whole cell lysates.
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Immunohistochemical analysis of LCK (Phospho-Y505) staining in human lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.