Description: Rabbit polyclonal antibody to Kir3.1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Kir3.1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: KCNJ3 Alternative Names: GIRK1; G protein-activated inward rectifier potassium channel 1; GIRK-1; Inward rectifier K(+) channel Kir3.1; Potassium channel, inwardly rectifying subfamily J member 3Entrez Gene (Human): 3760Entrez Gene (Mouse) : 16519Entrez Gene (Rat) : 50599SwissProt (Human): P48549SwissProt (Mouse) : P63250SwissProt (Rat) : P63251Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Kir3.1 expression in mouse brain (A) whole cell lysates.
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Immunofluorescent analysis of Kir3.1 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).