Description: Rabbit polyclonal antibody to CD213a1 (Phospho-Y405) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y405 of human CD213a1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: IL13RA1 Alternative Names: IL13R; IL13RA; Interleukin-13 receptor subunit alpha-1; IL-13 receptor subunit alpha-1; IL-13R subunit alpha-1; IL-13R-alpha-1; IL-13RA1; Cancer/testis antigen 19; CT19; CD213a1Entrez Gene (Human): 3597Entrez Gene (Mouse) : 16164SwissProt (Human): P78552SwissProt (Mouse) : O09030Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD213a1 (Phospho-Y405) expression in HCT116 (A), HuvEc (B) whole cell lysates.
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Immunofluorescent analysis of CD213a1 (Phospho-Y405) staining in HCT116 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).