Description: Rabbit polyclonal antibody to GDF9 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GDF9. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: GDF9 Alternative Names: Growth/differentiation factor 9; GDF-9Entrez Gene (Human): 2661Entrez Gene (Mouse) : 14566SwissProt (Human): O60383SwissProt (Mouse) : Q07105Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GDF9 expression in MCF7 (A), K562 (B), mouse muscle (C) whole cell lysates.
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Immunohistochemical analysis of GDF9 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.