Description: Rabbit polyclonal antibody to EPHA2 (Phospho-Y588/596) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y588/596 of human EPHA2 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: EPHA2; EPHA3; EPHA4 Alternative Names: EPHA2; ECK; Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; EPHA3; ETK; ETK1; HEK; TYRO4; Ephrin type-A receptor 3; EPH-like kinase 4; EK4; hEK4; HEK; Human embryo kinase; Tyrosine-protein kinase TYRO4; Tyrosine-protein kinase receptor ETK1; Eph-like tyrosine kinase 1; EPHA4; HEK8; SEK; TYRO1; Ephrin type-A receptor 4; EPH-like kinase 8; EK8; hEK8; Tyrosine-protein kinase TYRO1; Tyrosine-protein kinase receptor SEKEntrez Gene (Human): 1969; 2042; 2043Entrez Gene (Mouse) : 13836; 13838Entrez Gene (Rat) : 29210SwissProt (Human): P29317; P29320; P54764SwissProt (Mouse) : Q03145; P29319; Q03137SwissProt (Rat) : O08680Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of EPHA2 (Phospho-Y588/596) expression in A431 (A), mouse brain (B) whole cell lysates.
• 
  Immunofluorescent analysis of EPHA2 (Phospho-Y588/596) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).