Description: Rabbit polyclonal antibody to Alpha-crystallin B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Alpha-crystallin B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CRYAB Alternative Names: CRYA2; Alpha-crystallin B chain; Alpha(B)-crystallin; Heat shock protein beta-5; HspB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber componentEntrez Gene (Human): 1410Entrez Gene (Mouse) : 12955Entrez Gene (Rat) : 25420SwissProt (Human): P02511SwissProt (Mouse) : P23927SwissProt (Rat) : P23928Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Alpha-crystallin B expression in K562 (A) whole cell lysates.
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  Immunohistochemical analysis of Alpha-crystallin B staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.