Description: Rabbit polyclonal antibody to CREB Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CREB. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CREB1 Alternative Names: Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1Entrez Gene (Human): 1385Entrez Gene (Mouse) : 12912Entrez Gene (Rat) : 81646SwissProt (Human): P16220SwissProt (Mouse) : Q01147SwissProt (Rat) : P15337Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CREB expression in EC9706 (A), A549 (B), HEK293T (C) whole cell lysates.
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  Immunohistochemical analysis of CREB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.