Description: Rabbit polyclonal antibody to CHK1 (Phospho-S296) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S296 of human CHK1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CHEK1 Alternative Names: CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1Entrez Gene (Human): 1111Entrez Gene (Rat) : 140583SwissProt (Human): O14757SwissProt (Rat) : Q91ZN7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CHK1 (Phospho-S296) expression in U2OS (A), rat testis (B) whole cell lysates.
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  Immunohistochemical analysis of CHK1 (Phospho-S296) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.