Description: Rabbit polyclonal antibody to CDC25A (Phospho-S178) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S178 of human CDC25A protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CDC25A Alternative Names: M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25AEntrez Gene (Human): 993SwissProt (Human): P30304Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDC25A (Phospho-S178) expression in HEK293T UV-treated (A), HeLa UV-treated (B) whole cell lysates.
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Immunohistochemical analysis of CDC25A (Phospho-S178) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.