Description: Rabbit polyclonal antibody to Cyclin H (Phospho-T315) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T315 of human Cyclin H protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CCNH Alternative Names: Cyclin-H; MO15-associated protein; p34; p37Entrez Gene (Human): 902Entrez Gene (Mouse) : 66671Entrez Gene (Rat) : 84389SwissProt (Human): P51946SwissProt (Mouse) : Q61458SwissProt (Rat) : Q9R1A0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cyclin H (Phospho-T315) expression in HeLa (A), H1688 (B), mouse liver (C), rat liver (D) whole cell lysates.
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Immunohistochemical analysis of Cyclin H (Phospho-T315) staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.