Description: Rabbit polyclonal antibody to Calpain 11 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Calpain 11. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CAPN11 Alternative Names: Calpain-11; Calcium-activated neutral proteinase 11; CANP 11Entrez Gene (Human): 11131SwissProt (Human): Q9UMQ6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Calpain 11 expression in Hela (A), Jurkat (B) whole cell lysates.
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  Immunohistochemical analysis of Calpain 11 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.