Description: Rabbit polyclonal antibody to RhoGDI alpha (Phospho-S174) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S174 of human RhoGDI alpha protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ARHGDIA Alternative Names: GDIA1; Rho GDP-dissociation inhibitor 1; Rho GDI 1; Rho-GDI alphaEntrez Gene (Human): 396SwissProt (Human): P52565Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RhoGDI alpha (Phospho-S174) expression in HeLa (A), Jurkat (B) whole cell lysates.
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  Immunohistochemical analysis of RhoGDI alpha (Phospho-S174) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.