Description: Rabbit polyclonal antibody to Annexin A1 (Phospho-Y21) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y21 of human Annexin A1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ANXA1 Alternative Names: ANX1; LPC1; Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35Entrez Gene (Human): 301Entrez Gene (Mouse) : 16952Entrez Gene (Rat) : 25380SwissProt (Human): P04083SwissProt (Mouse) : P10107SwissProt (Rat) : P07150Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Annexin A1 (Phospho-Y21) expression in H1792 (A), mouse lung (B), rat lung (C) whole cell lysates.
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  Immunohistochemical analysis of Annexin A1 (Phospho-Y21) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.