Description: Rabbit polyclonal antibody to GRK2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GRK2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ADRBK1 Alternative Names: BARK; BARK1; GRK2; Beta-adrenergic receptor kinase 1; Beta-ARK-1; G-protein coupled receptor kinase 2Entrez Gene (Human): 156Entrez Gene (Rat) : 25238SwissProt (Human): P25098SwissProt (Mouse) : Q99MK8SwissProt (Rat) : P26817Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GRK2 expression in Jurkat (A), Raji (B), mouse kidney (C), rat kidney (D) whole cell lysates.
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Immunohistochemical analysis of GRK2 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.