Description: Rabbit polyclonal antibody to Aconitase 1 (Phospho-S711) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S711 of human Aconitase 1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ACO1 Alternative Names: IREB1; Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1Entrez Gene (Human): 48Entrez Gene (Mouse) : 11428Entrez Gene (Rat) : 50655SwissProt (Human): P21399SwissProt (Mouse) : P28271SwissProt (Rat) : Q63270Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Aconitase 1 (Phospho-S711) expression in Hela (A), HuvEc (B) whole cell lysates.
-
Immunohistochemical analysis of Aconitase 1 (Phospho-S711) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.