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Anti-Aconitase 1 (Phospho-S711) Antibody CPA4553
  • Western blot analysis of Aconitase 1 (Phospho-S711) expression in Hela (A), HuvEc (B) whole cell lysates.
  • Immunohistochemical analysis of Aconitase 1 (Phospho-S711) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-Aconitase 1 (Phospho-S711) Antibody
Cat No: CPA4553
Source: Rabbit
Reactivity: H, M, R, B, C, Rb, Z
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$420
100 μl
$260
30 μl
$130
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Description: Rabbit polyclonal antibody to Aconitase 1 (Phospho-S711)
Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S711 of human Aconitase 1 protein. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol: ACO1
Alternative Names: IREB1; Cytoplasmic aconitate hydratase; Aconitase; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1
Entrez Gene (Human): 48
Entrez Gene (Mouse) : 11428
Entrez Gene (Rat) : 50655
SwissProt (Human): P21399
SwissProt (Mouse) : P28271
SwissProt (Rat) : Q63270
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of Aconitase 1 (Phospho-S711) expression in Hela (A), HuvEc (B) whole cell lysates.
  • Immunohistochemical analysis of Aconitase 1 (Phospho-S711) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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