Description: Rabbit polyclonal antibody to cTnI (Phospho-S22/S23) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S22/S23 of human cTnI protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: TNNI3 Alternative Names: TNNI3; TNNC1; Troponin I, cardiac muscle; Cardiac troponin IEntrez Gene (Human): 7137Entrez Gene (Mouse) : 21954Entrez Gene (Rat) : 29248SwissProt (Human): P19429SwissProt (Mouse) : P48787SwissProt (Rat) : P23693Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of cTnI (Phospho-S22/S23) expression in mouse heart (A), moue kidney (B), rat heart (C), rat kidney (D) whole cell lysates.
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Immunohistochemical analysis of cTnI (Phospho-S22/S23) staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.