Description: Rabbit polyclonal antibody to SP1 (Phospho-T739) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T739 of human SP1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: SP1 Alternative Names: TSFP1; Transcription factor Sp1Entrez Gene (Human): 6667Entrez Gene (Mouse) : 20683Entrez Gene (Rat) : 24790SwissProt (Human): P08047SwissProt (Mouse) : O89090SwissProt (Rat) : Q01714Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SP1 (Phospho-T739) expression in H1688 (A) whole cell lysates.
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  Immunohistochemical analysis of SP1 (Phospho-T739) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.