Description: Rabbit polyclonal antibody to Ryanodine Receptor 2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Ryanodine Receptor 2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200) Gene Symbol: RYR2 Alternative Names: Ryanodine receptor 2; RYR-2; RyR2; hRYR-2; Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; Type 2 ryanodine receptorEntrez Gene (Human): 6262Entrez Gene (Mouse) : 20191Entrez Gene (Rat) : 689560SwissProt (Human): Q92736SwissProt (Mouse) : E9Q401SwissProt (Rat) : B0LPN4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Ryanodine Receptor 2 expression in PC3 (A), MCF7 (B), NIH3T3 (C), mouse brain (D) whole cell lysates.
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  Immunohistochemical analysis of Ryanodine Receptor 2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Ryanodine Receptor 2 staining in NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.