Description: Rabbit polyclonal antibody to RagA/B Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RagA/B. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: RRAGA; RRAGB Alternative Names: RRAGA; Ras-related GTP-binding protein A; Rag A; RagA; Adenovirus E3 14.7 kDa-interacting protein 1; FIP-1; RRAGB; Ras-related GTP-binding protein B; Rag B; RagBEntrez Gene (Human): 10670; 10325Entrez Gene (Mouse) : 68441; 245670Entrez Gene (Rat) : 117044; 100909655SwissProt (Human): Q7L523; Q5VZM2SwissProt (Mouse) : Q80X95; Q6NTA4SwissProt (Rat) : Q63486; Q63487Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RagA/B expression in mouse lung (A), rat testis (B) whole cell lysates.
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  Immunohistochemical analysis of RagA/B staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.