Description: Rabbit polyclonal antibody to PYK2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PYK2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: PTK2B Alternative Names: FAK2; PYK2; RAFTK; Protein-tyrosine kinase 2-beta; Calcium-dependent tyrosine kinase; CADTK; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; CAK-beta; CAKB; Focal adhesion kinase 2; FADK 2; Proline-rich tyrosine kinase 2; Related adhesion focal tyrosine kinase; RAFTKEntrez Gene (Human): 2185Entrez Gene (Mouse) : 19229Entrez Gene (Rat) : 50646SwissProt (Human): Q14289SwissProt (Mouse) : Q9QVP9SwissProt (Rat) : P70600Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PYK2 expression in Jurkat (A), K562 (B) whole cell lysates.
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Immunohistochemical analysis of PYK2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.