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Anti-PTEN Antibody CPA4496
  • Western blot analysis of PTEN expression in MCF7 (A), HepG2 (B) whole cell lysates.
  • Immunohistochemical analysis of PTEN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-PTEN Antibody
Cat No: CPA4496
Source: Rabbit
Reactivity: H, M, B, D, Mk, P, Z
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Rabbit polyclonal antibody to PTEN
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PTEN. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)
Gene Symbol: PTEN
Alternative Names: MMAC1; TEP1; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog
Entrez Gene (Human): 5728
Entrez Gene (Mouse) : 19211
SwissProt (Human): P60484
SwissProt (Mouse) : O08586
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of PTEN expression in MCF7 (A), HepG2 (B) whole cell lysates.
  • Immunohistochemical analysis of PTEN staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
SPOP suppresses tumorigenesis by regulating hedgehog/Gli2 signaling pathway in gastric cancer
Journal J Exp Clin Cancer Res
IF 11.3
Application
Reactivity Human
PMID 25204354
A novel PTEN gene promoter mutation and untypical Cowden syndrome
Journal Chin J Cancer Res
IF 5.1
Application
Reactivity
PMID 23825907
The oncogenic microRNA OncomiR-21 overexpressed during Marek's disease lymphomagenesis is transactivated by the viral oncoprotein Meq
Journal J Virol
IF 5.4
Application
Reactivity
PMID 23055556
Genomic Deletion of PTEN Is Associated with Tumor Progression and Early PSA Recurrence in ERG Fusion-Positive and Fusion-Negative Prostate Cancer
Journal Am J Pathol
IF 6
Application IHC-P
Reactivity Human
PMID 22705054
Simultaneous inactivation of Par-4 and PTEN in vivo leads to synergistic NF-kappaB activation and invasive prostate carcinoma
Journal Proc Natl Acad Sci U S A
IF
Application IHC-P
Reactivity Mouse
PMID 19470463
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