Description: Rabbit polyclonal antibody to PKC iota/zeta (Phospho-T412/410) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T412/410 of human PKC iota/zeta protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: PRKCI; PRKCZ Alternative Names: DXS1179E; Protein kinase C iota type; Atypical protein kinase C-lambda/iota; PRKC-lambda/iota; aPKC-lambda/iota; nPKC-iota; PKC2; Protein kinase C zeta type; nPKC-zetaEntrez Gene (Human): 5584; 5590Entrez Gene (Mouse) : 18759; 18762Entrez Gene (Rat) : 84006; 25522SwissProt (Human): P41743; Q05513SwissProt (Mouse) : Q62074; Q02956SwissProt (Rat) : F1M7Y5; P09217Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PKC iota/zeta (Phospho-T412/410) expression in Hela (A), mouse lung (B) whole cell lysates.
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  Immunohistochemical analysis of PKC iota/zeta (Phospho-T412/410) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of PKC iota/zeta (Phospho-T412/410) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.