Description: Rabbit polyclonal antibody to PI3K p85 alpha/p55 gamma (Phospho-Y467/199) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y467/199 of human PI3K p85 alpha/p55 gamma protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: PIK3R1; PIK3R3 Alternative Names: PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; PIK3R3; Phosphatidylinositol 3-kinase regulatory subunit gamma; PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma; p55PIKEntrez Gene (Human): 5295Entrez Gene (Mouse) : 18708; 18710Entrez Gene (Rat) : 25513; 60664SwissProt (Human): P27986; Q92569SwissProt (Mouse) : P26450; Q64143SwissProt (Rat) : Q63787; Q63789Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PI3K p85 alpha/p55 gamma (Phospho-Y467/199) expression in Jurkat (A), MCF7 (B), mouse kidney (C), mouse muscle (D), rat kidney (E), rat muscle (F) whole cell lysates. (Predicted band size: 83; 54 kD; Observed band size: 85 kD)
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  Immunohistochemical analysis of PI3K p85 alpha/p55 gamma (Phospho-Y467/199) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of PI3K p85 alpha/p55 gamma (Phospho-Y467/199) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).