Description: Rabbit polyclonal antibody to NEFL Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NEFL. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: NEFL Alternative Names: NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L proteinEntrez Gene (Human): 4747Entrez Gene (Mouse) : 18039Entrez Gene (Rat) : 83613SwissProt (Human): P07196SwissProt (Mouse) : P08551SwissProt (Rat) : P19527Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NEFL expression in Jurkat (A), NIH3T3 (B) whole cell lysates.
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Immunohistochemical analysis of NEFL staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.