Description: Rabbit polyclonal antibody to MEF2D (Phospho-S444) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S444 of human MEF2D protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: MEF2D Alternative Names: Myocyte-specific enhancer factor 2DEntrez Gene (Human): 4209Entrez Gene (Mouse) : 17261Entrez Gene (Rat) : 81518SwissProt (Human): Q14814SwissProt (Mouse) : Q63943SwissProt (Rat) : O89038Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of MEF2D (Phospho-S444) expression in HEK293T (A), Jurkat (B), U2OS (C) whole cell lysates.
-
Immunohistochemical analysis of MEF2D (Phospho-S444) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.