Description: Rabbit polyclonal antibody to Histone Deacetylase 5/9 (Phospho-S259/220) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S259/220 of human Histone Deacetylase 5/9 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: HDAC5; HDAC9 Alternative Names: HDAC5; KIAA0600; Histone deacetylase 5; HD5; Antigen NY-CO-9; HDAC9; HDAC7; HDAC7B; HDRP; KIAA0744; MITR; Histone deacetylase 9; HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription repressor MITREntrez Gene (Human): 10014; 9734Entrez Gene (Mouse) : 79221SwissProt (Human): Q9UQL6; Q9UKV0SwissProt (Mouse) : Q9Z2V6; Q99N13Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone Deacetylase 5/9 (Phospho-S259/220) expression in PC3 (A), HeLa (B), NIH3T3 (C) whole cell lysates.
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  Immunohistochemical analysis of Histone Deacetylase 5/9 (Phospho-S259/220) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.