Description: Rabbit polyclonal antibody to Ephrin B1/2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Ephrin B1/2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: EFNB1; EFNB2 Alternative Names: EFNB1; EFL3; EPLG2; LERK2; Ephrin-B1; EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2; EFNB2; EPLG5; HTKL; LERK5; Ephrin-B2; EPH-related receptor tyrosine kinase ligand 5; LERK-5; HTK ligand; HTK-LEntrez Gene (Human): 1947; 1948Entrez Gene (Mouse) : 13641; 13642SwissProt (Human): P98172; P52799SwissProt (Mouse) : P52795; P52800SwissProt (Rat) : P52796Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Ephrin B1/2 expression in mouse heart (A), rat brain (B), U87MG (C) whole cell lysates.
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  Immunohistochemical analysis of Ephrin B1/2 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.