Description: Rabbit polyclonal antibody to EEF2K Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EEF2K. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: EEF2K Alternative Names: Eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinaseEntrez Gene (Human): 101930123; 29904Entrez Gene (Mouse) : 13631Entrez Gene (Rat) : 25435SwissProt (Human): O00418SwissProt (Mouse) : O08796SwissProt (Rat) : P70531Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of EEF2K expression in MCF7 (A) whole cell lysates.
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Immunohistochemical analysis of EEF2K staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.