Description: Rabbit polyclonal antibody to DDX52 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DDX52. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: DDX52 Alternative Names: ROK1; Probable ATP-dependent RNA helicase DDX52; ATP-dependent RNA helicase ROK1-like; DEAD box protein 52Entrez Gene (Human): 11056SwissProt (Human): Q9Y2R4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DDX52 expression in HEK293T (A), Hela (B), HGC27 (C), mouse kidney (D), mouse liver (E), rat kidney (F), rat liver (G) whole cell lysates.
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  Immunohistochemical analysis of DDX52 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.