Description: Rabbit polyclonal antibody to IKK alpha/beta (Phospho-S176/177) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S176/177 of human IKK alpha/beta protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CHUK; IKBKB Alternative Names: CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16; IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKBEntrez Gene (Human): 1147; 3551Entrez Gene (Mouse) : 16150Entrez Gene (Rat) : 84351SwissProt (Human): O15111; O14920SwissProt (Mouse) : Q60680; O88351SwissProt (Rat) : Q9QY78Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IKK alpha/beta (Phospho-S176/177) expression in HeLa LPS-treated (A), mouse brain (B), rat brain (C) whole cell lysates.
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Immunohistochemical analysis of IKK alpha/beta (Phospho-S176/177) staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.