Description: Rabbit polyclonal antibody to CDC6 (Phospho-S54) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S54 of human CDC6 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CDC6 Alternative Names: CDC18L; Cell division control protein 6 homolog; CDC6-related protein; Cdc18-related protein; HsCdc18; p62(cdc6); HsCDC6Entrez Gene (Human): 990Entrez Gene (Mouse) : 23834SwissProt (Human): Q99741SwissProt (Mouse) : O89033Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CDC6 (Phospho-S54) expression in HeLa serum starvation-treated (A), Raw264.7 serum starvation-treated (B), H9C2 serum starvation-treated (C) whole cell lysates.
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  Immunohistochemical analysis of CDC6 (Phospho-S54) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.