Description: Rabbit polyclonal antibody to Cyclin D1 (Phospho-T286) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T286 of human Cyclin D1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CCND1 Alternative Names: BCL1; PRAD1; G1/S-specific cyclin-D1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogeneEntrez Gene (Human): 595Entrez Gene (Mouse) : 12443Entrez Gene (Rat) : 58919SwissProt (Human): P24385SwissProt (Mouse) : P25322SwissProt (Rat) : P39948Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cyclin D1 (Phospho-T286) expression in MCF7 EGF-treated (A), rat muscle (B) whole cell lysates.
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Immunohistochemical analysis of Cyclin D1 (Phospho-T286) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Evidence for cell cycle suppression and microRNA regulation of cyclin D1 during anoxia exposure in turtles