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Anti-ATP1A1 (Phospho-S16) Antibody CPA4339
  • Western blot analysis of ATP1A1 (Phospho-S16) expression in HEK293T (A), HCT116 (B), Panc1 (C), PC12 (D), CT26 (E) whole cell lysates.
  • Immunohistochemical analysis of ATP1A1 (Phospho-S16) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product NameAnti-ATP1A1 (Phospho-S16) Antibody
Cat No: CPA4339
Source: Rabbit
Reactivity: H, M, R, B, D, P, Rb, S
Applications: WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$420
100 μl
$260
30 μl
$130
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Description: Rabbit polyclonal antibody to ATP1A1 (Phospho-S16)
Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S16 of human ATP1A1 protein. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100)
Gene Symbol: ATP1A1
Alternative Names: Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1
Entrez Gene (Human): 476
Entrez Gene (Mouse) : 11928
Entrez Gene (Rat) : 24211
SwissProt (Human): P05023
SwissProt (Mouse) : Q8VDN2
SwissProt (Rat) : P06685
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of ATP1A1 (Phospho-S16) expression in HEK293T (A), HCT116 (B), Panc1 (C), PC12 (D), CT26 (E) whole cell lysates.
  • Immunohistochemical analysis of ATP1A1 (Phospho-S16) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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